On a certain continent, nearly all kingdoms worship under the same pantheon. However, in the southern reaches the peoples take a much more...liberal stance on their Gods. Statues are nude, and very anatomically correct, and icons are often startlingly brazen. For instance, the icon of (insert name), the goddess of love, is an image of two nude twins embracing in a passionate kiss, signifying the love of both family and partner. This is a source of unending outrage and offense for the Northern churches, whose traditional and modest take on religion is constantly at odds with the near-blasphemous ideals of the Southerners. While this is not enough to provoke outright conflict, there is more than enough simmering discontent and long-held grudges between the two hemispheres.
Pirates' many bejeweled rings and piercings actually had a practical purpose - when the pirate or sailor died, the rings could be taken off as payment for a proper burial, saving him from a watery grave. This could be tied into regional culture, or made into a quest (The Pirate's Lost Rings, etc.). Also gives treasure-seeking divers another thing to look for besides crusty old chests.
A group of humans living in a mountainous area have spent generations mining, drinking home made liquor, and generally not spreading the gene pool around enough. The end result is a sub-race of humans who no longer have necks, rather their heads protrude from the upper portion of the torso between the shoulders. They have beards, and lacking the ability to turn their heads, can only see what they are directly facing. They are simple and to the point, and direct to the point of bluntness.