This is an excellent adventure idea that can be readily changed to fit almost any setting. I think there has even been Dr. Who episodes that have a similar theme - i'm talking old, single-digit Doctors.
You type the command into the console. "Insufficient Guineapigs" the computer responds. Baffled, you retype the command. "Insufficient Guineapigs" the computer replies again. You decide you should start smaller, with a simpler command. "Insufficient Guineapigs"
What does cycling the spaceship's airlock have to do with a now-extinct Peruvian rodent?